ELISA with Biotinylated Human EGFRVIII Protein 4425

Activity assay with Biotinylated Human EGFRVIII Protein 4425

Biotinylated Human EGFRVIII Protein 4425

$525.00 – $1,750.00

Summary

Expression: HEK293
Active: Yes (catalytic)
Amino Acid Range: Leu25-Ser378

SKU: 4425parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
molecular weight	The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 60-78 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human EGFRVIII Protein 4425

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

Data

ELISA with Biotinylated Human EGFRVIII Protein

Immobilized Biotinylated Human EGFRVIII, His Tag at 0.5µg/ml (100µl/Well) on streptavidin (5µg/ml) precoated plate. Dose response curve for Anti-EGFR VIII Anitibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).

Activity assay with Biotinylated Human EGFRVIII Protein

Use Biotinylated Human EGFRVIII, His Tag protein to detect the expression rate of Anti-EGFRVIII-CAR positive cell. 293T cells and anti-EGFRVIII CAR-293T cells were incubated with Biotinylated human EGFR VIII-His Tag. Non-transfected 293T cells and Biotinylated protein control were used as negative control. SA-PE was used to evaluate the binding activity of Biotinylated Human EGFR VIII.

HPLC of Biotinylated Human EGFRVIII Protein

The purity of Biotinylated Human EGFRVIII is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human EGFRVIII Protein

Biotinylated Human EGFRVIII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.