Skip to content

Biotinylated Human E-Selectin/CD62E Protein 5205

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Trp22-Pro556
SKU: 5205parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 – 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

molecular weight

The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Biotinylated Human E-Selectin/CD62E Protein 5205

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Protein names
E-selectin (CD62 antigen-like family member E) (Endothelial leukocyte adhesion molecule 1) (ELAM-1) (Leukocyte-endothelial cell adhesion molecule 2) (LECAM2) (CD antigen CD62E)
Gene names
SELE,SELE ELAM1
Protein family
Selectin/LECAM family
Mass
9606Da
Function
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Catalytic activity
BINDING 101..109; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269|PubMed:26117840, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 101; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:26117840, ECO:0007744|PDB:1ESL, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 103; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:26117840, ECO:0007744|PDB:1ESL, ECO:0007744|PDB:1G1T, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 109; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:26117840, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 113..118; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269|PubMed:26117840, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 126..128; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269|PubMed:26117840, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 126; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:26117840, ECO:0007744|PDB:1ESL, ECO:0007744|PDB:1G1T, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 127; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:26117840, ECO:0007744|PDB:1ESL, ECO:0007744|PDB:1G1T, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"
Subellular location
Cell membrane ; Single-pass type I membrane protein.
Structure
Interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope. SELPLG sulfation appears not to be required for this interaction.
Target Relevance information above includes information from UniProt accession: P16581
The UniProt Consortium

Data

ELISA with Biotinylated Human E-Selectin/CD62E Protein
Immobilized Biotinylated Human E-selectin, His Tag at 1µg/ml (100µl/Well) on streptavidin (5µg/ml) precoated plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
HPLC of Biotinylated Human E-Selectin/CD62E Protein
The purity of Biotinylated Human E-selectin is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human E-Selectin/CD62E Protein
Biotinylated Human E-selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product

Documents

#
Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information.

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.