SPR with Biotinylated Human DNAM-1/CD226 Protein 4162

ELISA with Biotinylated Human DNAM-1/CD226 Protein 4162

Biotinylated Human DNAM-1/CD226 Protein 4162

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Glu19-Asn247

SKU: 4162parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
background	DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
molecular weight	The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human DNAM-1/CD226 Protein 4162

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
Protein names CD226 antigen (DNAX accessory molecule 1) (DNAM-1) (CD antigen CD226)
Gene names CD226,CD226 DNAM1
Mass 9606Da
Function Involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell (PubMed:8673704). Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding (PubMed:26755705).
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Expressed by peripheral blood T-lymphocytes.
Structure Interacts with PVR and NECTIN2. Competes with PVRIG for NECTIN2-binding.
Target Relevance information above includes information from UniProt accession: Q15762
The UniProt Consortium

Data

SPR with Biotinylated Human DNAM-1/CD226 Protein

Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human DNAM-1, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay (Biacore T200).

ELISA with Biotinylated Human DNAM-1/CD226 Protein

Immobilized Biotinylated Human DNAM-1, His Tag at 1µg/ml (100µl/Well) on the streptavidin precoated plate(5µg/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test).

HPLC of Biotinylated Human DNAM-1/CD226 Protein

The purity of Biotinylated Human DNAM-1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human DNAM-1/CD226 Protein

Biotinylated Human DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.