ELISA with Biotinylated Human CLEC12A/MICL/CLL-1 Protein 4176

Biotinylated Human CLEC12A/MICL/CLL-1 Protein 4176

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: His65-Ala265

SKU: 4176parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	N-His, C-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML.
molecular weight	The protein has a predicted MW of 26.4 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human CLEC12A/MICL/CLL-1 Protein 4176

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML.
Protein names C-type lectin domain family 12 member A (C-type lectin-like molecule 1) (CLL-1) (Dendritic cell-associated lectin 2) (DCAL-2) (Myeloid inhibitory C-type lectin-like receptor) (MICL) (CD antigen CD371)
Gene names CLEC12A,CLEC12A CLL1 DCAL2 MICL
Mass 9606Da
Function Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases.
Subellular location Cell membrane ; Single-pass type II membrane protein. Note=Ligand binding leads to internalization.
Tissues Detected in normal myeloid cells and in acute myeloid leukemia cells. Detected in neutrophils, eosinophils, monocytes and dendritic cells. Detected in spleen macrophage-rich red pulp and in lymph node (at protein level). Detected in peripheral blood leukocytes, dendritic cells, bone marrow, monocytes, mononuclear leukocytes and macrophages.
Structure Interacts with PTPN6 and PTPN11.
Post-translational modification Highly N-glycosylated. Glycosylation varies between cell types.
Domain Co
Target Relevance information above includes information from UniProt accession: Q5QGZ9
The UniProt Consortium

Data

ELISA with Biotinylated Human CLEC12A/MICL/CLL-1 Protein

Immobilized Biotinylated Human CLEC12A, His Tag at 1µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Anti-CLEC12A Antibody, hFc Tag with the EC50 of 29.6ng/ml determined by ELISA.

HPLC of Biotinylated Human CLEC12A/MICL/CLL-1 Protein

The purity of Biotinylated Human CLEC12A is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human CLEC12A/MICL/CLL-1 Protein

Biotinylated Human CLEC12A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.