Biotinylated Human Claudin 6 Protein-VLP 4250

$1,969.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Met1-Val220

SKU: 4250parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P56747
express system	HEK293
product tag
purity	> 95% as determined by HPLC
background	Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.
molecular weight	The target protein has a predicted MW of 24.5 kDa.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μl by the LAL method.

Biotinylated Human Claudin 6 Protein-VLP 4250

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.
Protein names Claudin-6 (Skullin)
Gene names CLDN6,CLDN6 UNQ757/PRO1488
Protein family Claudin family
Mass 9606Da
Function Plays a major role in tight junction-specific obliteration of the intercellular space.; (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.
Subellular location Cell junction, tight junction. Cell membrane ; Multi-pass membrane protein .
Tissues Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines.
Structure Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity). Interacts with CLDN1, CD81 and OCLN (PubMed:20375010).
Target Relevance information above includes information from UniProt accession: P56747
The UniProt Consortium

Data

SPR with Biotinylated Human Claudin 6 Protein-VLP

Biotinylated Human Claudin 6 VLP captured on CM5 Chip via Streptavidin can bind Anti-Claudin6 Antibody with an affinity constant of 0.65 nM as determined in SPR assay (Biacore T200).

HPLC of Biotinylated Human Claudin 6 Protein-VLP

The purity of Biotinylated Human Claudin 6 VLP is greater than 95% as determined by SEC-HPLC.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.