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Biotinylated Human Claudin 18.2 Protein-VLP 4901

$1,688.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Met1-Val261
SKU: 4901parent Categories: , Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P56856

product tag

tagless, biotinylated

express system

HEK293

purity

> 95% as determined by HPLC

background

Claudin18(CLDN18) belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells.CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. Isoform 2 (Claudin 18.2) is abundant in gastric tumors.

molecular weight

The protein has a predicted MW of 29 kDa.

available size

100 µL

endotoxin

Less than 1EU per μg by the LAL method.

Biotinylated Human Claudin 18.2 Protein-VLP 4901

protein
Size and concentration
100, 500µg and liquid
Form
Liquid
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped with dry ice.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Claudin18(CLDN18) belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells.CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants,CLDN18.1 and CLDN18.2. Isoform 2 (Claudin 18.2) is abundant in gastric tumors.
Protein names
Claudin-18
Gene names
CLDN18,CLDN18 UNQ778/PRO1572
Protein family
Claudin family
Mass
9606Da
Function
FUNCTION: Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling (By similarity). Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier (By similarity). Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription (By similarity). Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency (By similarity). Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways (By similarity). {ECO:0000250|UniProtKB:P56857}.; FUNCTION: [Isoform A1]: Involved in the maintenance of homeostasis of the alveolar microenvironment via regulation of pH and subsequent T-cell activation in the alveolar space, is therefore indirectly involved in limiting C. neoformans infection. {ECO:0000250|UniProtKB:P56857}.; FUNCTION: [Isoform A2]: Required for the formation of the gastric paracellular barrier via its role in tight junction formation, thereby involved in the response to gastric acidification. {ECO:0000250|UniProtKB:P56857}.
Subellular location
SUBCELLULAR LOCATION: Cell junction, tight junction {ECO:0000250|UniProtKB:P56857}. Cell membrane {ECO:0000250|UniProtKB:P56857}; Multi-pass membrane protein {ECO:0000255}. Note=Localizes to tight junctions in epithelial cells. {ECO:0000250|UniProtKB:P56857}.; SUBCELLULAR LOCATION: [Isoform A1]: Cell junction, tight junction {ECO:0000250|UniProtKB:P56857}.; SUBCELLULAR LOCATION: [Isoform A2]: Cell junction, tight junction {ECO:0000250|UniProtKB:P56857}. Lateral cell membrane {ECO:0000250|UniProtKB:P56857}.
Tissues
TISSUE SPECIFICITY: [Isoform A1]: Expression is restricted to the lung. {ECO:0000269|PubMed:19047087}.; TISSUE SPECIFICITY: [Isoform A2]: Expression is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine cells (at protein level). {ECO:0000269|PubMed:19047087}.
Structure
SUBUNIT: Interacts with TJP2/ZO-2 (By similarity). Interacts with TJP1/ZO-1 (By similarity). Interacts with YAP1 (phosphorylated); the interaction sequesters YAP1 away from the nucleus and thereby restricts transcription of YAP1 target genes (By similarity). {ECO:0000250|UniProtKB:P56857}.
Target Relevance information above includes information from UniProt accession : P56856
The UniProt Consortium

Data

HPLC of Biotinylated Human Claudin 18.2 Protein-VLP
The purity of Biotinylated Human Claudin 18.2 VLP is greater than 95% as determined by SEC-HPLC.
ELISA with Biotinylated Human Claudin 18.2 Protein-VLP
Immobilized Biotinylated Human Claudin 18.2 VLP on streptavidin (5µg/ml) precoated plate. Dose response curve for Anti-Claudin18.2 Antibody, hFc Tag with the EC50 of 62ng/ml determined by ELISA.
SPR with Biotinylated Human Claudin 18.2 Protein-VLP
Amine coupling SA on CM5 Chip, SA capture Biotinylated Human Claudin 18.2 VLP, Anti-Claudin18.2 Ab. as analyte; The affinity constant of 7.74 nM as determined in SPR assay (Biacore T200).

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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