ELISA with Biotinylated Human CD5 Protein 4678

Biotinylated Human CD5 Protein 4678

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Arg25-Asn371

SKU: 4678parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-hFc-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
molecular weight	The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human CD5 Protein 4678

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
Protein names T-cell surface glycoprotein CD5 (Lymphocyte antigen T1/Leu-1) (CD antigen CD5)
Gene names CD5,CD5 LEU1
Mass 9606Da
Function May act as a receptor in regulating T-cell proliferation.
Subellular location Cell membrane; Single-pass type I membrane protein.
Structure Interacts with CD72/LYB-2. Interacts with PTPN6/SHP-1 (By similarity).
Post-translational modification Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1.
Target Relevance information above includes information from UniProt accession: P06127
The UniProt Consortium

Data

ELISA with Biotinylated Human CD5 Protein

Immobilized Anti-CD5 Antibody, hFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.

The purity of Biotinylated Human CD5 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human CD5 Protein

Biotinylated Human CD5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.