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Biotinylated Human CD300A Protein 2373

$600.00$2,000.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Leu18-Pro180
SKU: 2373parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
express system

HEK293

product tag

C-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation.

molecular weight

The protein has a predicted MW of 19.46 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Biotinylated Human CD300A Protein 2373

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation.
Protein names
CMRF35-like molecule 8 (CLM-8) (CD300 antigen-like family member A) (CMRF-35-H9) (CMRF35-H9) (CMRF35-H) (IRC1/IRC2) (Immunoglobulin superfamily member 12) (IgSF12) (Inhibitory receptor protein 60) (IRp60) (NK inhibitory receptor) (CD antigen CD300a)
Gene names
CD300A,CD300A CMRF35H IGSF12 HSPC083
Protein family
CD300 family
Mass
9606Da
Function
Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation (PubMed:10746781, PubMed:16339535, PubMed:9701027). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6 (PubMed:22043923).
Subellular location
Cell membrane ; Single-pass type I membrane protein .
Tissues
Expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.
Structure
Upon tyrosine-phosphorylation, interacts with PTN6/SHP-1 and PTPN11/SHP-2 and INPP5D.
Post-translational modification
Phosphorylated on tyrosine.; N-glycosylated.
Target Relevance information above includes information from UniProt accession: Q9UGN4
The UniProt Consortium

ELISA with Biotinylated Human CD300A Protein
Immobilized Biotinylated Human CD300A, Avi Tag at 0.5µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA.
HPLC of Biotinylated Human CD300A Protein
The purity of Biotinylated Human CD300A is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human CD300A Protein
Biotinylated Human CD300A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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