Biotinylated Human CD27/TNFRSF7 Protein 2024

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Thr21-Ile192

SKU: 2024parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-hFc-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
molecular weight	The protein has a predicted MW of 47.01 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human CD27/TNFRSF7 Protein 2024

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co-stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
Protein names CD27 antigen (CD27L receptor) (T-cell activation antigen CD27) (T14) (Tumor necrosis factor receptor superfamily member 7) (CD antigen CD27)
Gene names CD27,CD27 TNFRSF7
Mass 9606Da
Function Receptor specifically expressed at the surface of T cells which binds and is activated by its ligand CD70/CD27L expressed by B cells (PubMed:28011863). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed:28011863).
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Found in most T-lymphocytes.
Structure Homodimer (PubMed:1655907, PubMed:34419446). Interacts with SIVA1; may play a role in apoptosis through association with SIVA1 (PubMed:9177220). Interacts with TRAF2 (PubMed:9692890).
Post-translational modification Phosphorylated.; N-glycosylated.; O-glycosylated with core 1 or possibly core 8 glycans.
Target Relevance information above includes information from UniProt accession: P26842
The UniProt Consortium

ELISA with Biotinylated Human CD27/TNFRSF7 Protein

Immobilized Human CD27 Ligand (Trimer), His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 81.4ng/ml determined by ELISA.

HPLC of Biotinylated Human CD27/TNFRSF7 Protein

The purity of Biotinylated Human CD27 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human CD27/TNFRSF7 Protein

Biotinylated Human CD27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.