ELISA with Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary 4179

HPLC of Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Ami 4179

SDS-PAGE gel of Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Pri 4179

Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) 4179

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Leu50-Pro193

SKU: 4179parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	N-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7. CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
molecular weight	The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) 4179

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.Â CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Protein names CD70 antigen (CD27 ligand) (CD27-L) (Tumor necrosis factor ligand superfamily member 7) (CD antigen CD70)
Gene names CD70,CD70 CD27L CD27LG TNFSF7
Protein family Tumor necrosis factor family
Mass 9606Da
Function Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed:28011863, PubMed:28011864, PubMed:8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed:28011863, PubMed:28011864).
Subellular location Cell membrane ; Single-pass type II membrane protein .
Structure Homotrimer.
Post-translational modification N-glycosylated.
Target Relevance information above includes information from UniProt accession: P32970
The UniProt Consortium

Data

ELISA with Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary

Immobilized Human CD27, hFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human CD27 Ligand (Trimer) , His Tag with the EC50 of 9.2ng/ml determined by ELISA.

HPLC of Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Ami

The purity of Biotinylated Human CD27 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Pri

Biotinylated Human CD27 Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.