ELISA with Biotinylated Human B7-H7/HHLA2 Protein 2121

Biotinylated Human B7-H7/HHLA2 Protein 2121

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ile23-Asn344

SKU: 2121parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
molecular weight	The protein has a predicted MW of 39.88 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human B7-H7/HHLA2 Protein 2121

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
Protein names HERV-H LTR-associating protein 2 (Human endogenous retrovirus-H long terminal repeat-associating protein 2)
Mass 46850Da
Function Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade. {ECO:0000269\|PubMed:23784006}.
Subellular location Membrane {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}.
Tissues Expressed at high levels in colon, kidney, testis, lung and pancreas, and at lower levels in small intestine, liver and skeletal muscle. In immune cells, highly expressed in B-cells, dendritic cells and macrophages. Not detected in T-cells. {ECO:0000269\|PubMed:10444326, ECO:0000269\|PubMed:23784006}.
Structure Interacts with TMIGD2. {ECO:0000269\|PubMed:23784006}.
Target Relevance information above includes information from UniProt accession: Q9UM44
The UniProt Consortium

ELISA with Biotinylated Human B7-H7/HHLA2 Protein

Immobilized Biotinylated Human B7-H7, His Tag at 1µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Human CD28H, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.

HPLC of Biotinylated Human B7-H7/HHLA2 Protein

The purity of Biotinylated Human B7-H7 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human B7-H7/HHLA2 Protein

Biotinylated Human B7-H7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.