Skip to content

Biotinylated Human ANGPT2/Angiopoietin-2 Protein 5210

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Lys275-Phe496
Weight1 lbs
Dimensions9 × 5 × 2 in
product tag

N-His-Avi, also biotinylated

Biotinylated Human ANGPT2/Angiopoietin-2 Protein 5210

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.
Protein names
Angiopoietin-2 (ANG-2)
Gene names
ANGPT2,ANGPT2
Mass
9606Da
Function
FUNCTION: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (PubMed:15284220, PubMed:19116766, PubMed:19223473, PubMed:9204896). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (PubMed:15284220, PubMed:19116766, PubMed:19223473, PubMed:9204896). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (PubMed:15284220, PubMed:19116766, PubMed:19223473, PubMed:9204896). Involved in the regulation of lymphangiogenesis (PubMed:32908006). {ECO:0000269|PubMed:15284220, ECO:0000269|PubMed:19116766, ECO:0000269|PubMed:19223473, ECO:0000269|PubMed:32908006, ECO:0000269|PubMed:9204896}.
Subellular location
SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:32908006}.
Structure
SUBUNIT: Interacts with TEK/TIE2, competing for the same binding site as ANGPT1 (PubMed:12427764, PubMed:15284220, PubMed:19223473, PubMed:32908006, PubMed:9204896). Interacts with ITGA5 (PubMed:32908006). Interacts with SVEP1/polydom (By similarity). {ECO:0000250|UniProtKB:O35608, ECO:0000269|PubMed:12427764, ECO:0000269|PubMed:15284220, ECO:0000269|PubMed:16732286, ECO:0000269|PubMed:19223473, ECO:0000269|PubMed:32908006, ECO:0000269|PubMed:9204896}.
Domain
DOMAIN: Th
Target Relevance information above includes information from UniProt accession : O15123
The UniProt Consortium

Data

SPR with Biotinylated Human ANGPT2/Angiopoietin-2 Protein
Human TIE2, His Tag immobilized on CM5 Chip can bind Biotinylated Human ANGPT2, His Tag with an affinity constant of 2.93 µM as determined in SPR assay (Biacore T200).
ELISA with Biotinylated Human ANGPT2/Angiopoietin-2 Protein
Immobilized Biotinylated Human ANGPT2, His Tag at 1µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
SDS-PAGE gel of Biotinylated Human ANGPT2/Angiopoietin-2 Protein
Biotinylated Human ANGPT2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product

Documents

#
No results found

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.