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Biotinylated Human Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) 2179

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Ile23-Asp506
SKU: 2179parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

molecular weight

The protein has a predicted MW of 53.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Biotinylated Human Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) 2179

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Protein names
Alkaline phosphatase, placental type (EC 3.1.3.1) (Alkaline phosphatase Regan isozyme) (Placental alkaline phosphatase 1) (PLAP-1)
Gene names
ALPP,ALPP PLAP
Protein family
Alkaline phosphatase family
Mass
9606Da
Function
Alkaline phosphatase that can hydrolyze various phosphate compounds.
Catalytic activity
BINDING 64; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000269|PubMed:11124260, ECO:0000269|PubMed:15946677, ECO:0000269|PubMed:16815919, ECO:0000269|PubMed:20693656"; BINDING 64; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000269|PubMed:11124260, ECO:0000269|PubMed:15946677, ECO:0000269|PubMed:16815919, ECO:0000269|PubMed:20693656"; BINDING 114; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000269|PubMed:11124260, ECO:0000269|PubMed:15946677, ECO:0000269|PubMed:16815919, ECO:0000269|PubMed:20693656"; BINDING 177; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000269|PubMed:11124260, ECO:0000269|PubMed:15946677, ECO:0000269|PubMed:16815919, ECO:0000269|PubMed:20693656"; BINDING 238; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:P05186"; BINDING 291; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:P05186"; BINDING 292; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:P05186"; BINDING 307; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:P05186"; BINDING 333; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000269|PubMed:11124260, ECO:0000269|PubMed:15946677, ECO:0000269|PubMed:16815919, ECO:0000269|PubMed:20693656"; BINDING 338; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000269|PubMed:11124260, ECO:0000269|PubMed:15946677, ECO:0000269|PubMed:16815919, ECO:0000269|PubMed:20693656"; BINDING 342; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000269|PubMed:11124260, ECO:0000269|PubMed:15946677, ECO:0000269|PubMed:16815919, ECO:0000269|PubMed:20693656"; BINDING 379; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000269|PubMed:11124260, ECO:0000269|PubMed:15946677, ECO:0000269|PubMed:16815919, ECO:0000269|PubMed:20693656"; BINDING 380; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000269|PubMed:11124260, ECO:0000269|PubMed:15946677, ECO:0000269|PubMed:16815919, ECO:0000269|PubMed:20693656"; BINDING 454; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000269|PubMed:11124260, ECO:0000269|PubMed:15946677, ECO:0000269|PubMed:16815919, ECO:0000269|PubMed:20693656"
Subellular location
Cell membrane; Lipid-anchor, GPI-anchor .
Tissues
Detected in placenta (at protein level).
Structure
Homodimer.
Target Relevance information above includes information from UniProt accession: P05187
The UniProt Consortium

ELISA with Biotinylated Human Alkaline Phosphatase (Placental type) /AL
Immobilized Biotinylated Human Alkaline Phosphatase, His Tag at 0.5µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
HPLC of Biotinylated Human Alkaline Phosphatase (Placental type) /ALPP
The purity of Biotinylated Human Alkaline Phosphatase (Placental type) is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human Alkaline Phosphatase (Placental type
Biotinylated Human Alkaline Phosphatase (Placental type) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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