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Biotinylated Human Activin RIIB/ACVR2B Protein 2160

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Ser19-Thr137
SKU: 2160parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9).

molecular weight

The protein has a predicted MW of 16.56 kDa. Due to glycosylation, the protein migrates to 33-43 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Biotinylated Human Activin RIIB/ACVR2B Protein 2160

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9).
Protein names
Activin receptor type-2B (EC 2.7.11.30) (Activin receptor type IIB) (ACTR-IIB)
Gene names
ACVR2B,ACVR2B
Protein family
Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfam
Mass
9606Da
Function
Transmembrane serine/threonine kinase activin type-2 receptor forming an activin receptor complex with activin type-1 serine/threonine kinase receptors (ACVR1, ACVR1B or ACVR1c). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, the type-2 receptors act as a primary activin receptors (binds activin-A/INHBA, activin-B/INHBB as well as inhibin-A/INHA-INHBA). The type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor.
Subellular location
Cell membrane ; Single-pass type I membrane protein .
Structure
Forms an activin receptor complex with activin type II receptors such as ACVR1B. Interacts with VPS39. Interacts with DYNLT1. Interacts with BMP3 (PubMed:31665064). Interacts with BMP2 (PubMed:17295905). Interacts with BMP6 (PubMed:18070108).
Post-translational modification
Phosphorylated. Constitutive phosphorylation is in part catalyzed by its own kinase activity.
Target Relevance information above includes information from UniProt accession: Q13705
The UniProt Consortium

ELISA with Biotinylated Human Activin RIIB/ACVR2B Protein
Immobilized Human Activin A, No Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human Activin RIIB, His Tag with the EC50 of 53.8ng/ml determined by ELISA (QC Test).
ELISA with Biotinylated Human Activin RIIB/ACVR2B Protein
Immobilized Biotinylated Human Activin RIIB, His Tag at 0.5µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Anti-Activin RIIB Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
HPLC of Biotinylated Human Activin RIIB/ACVR2B Protein
The purity of Biotinylated Human Activin RIIB is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human Activin RIIB/ACVR2B Protein
Biotinylated Human Activin RIIB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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