Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein 5227

Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein 5227

$600.00 – $2,000.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln20-Glu351

SKU: 5227parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	N-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
molecular weight	The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein 5227

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

Data

ELISA with Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein

Immobilized Cynomolgus GDF15, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA.

HPLC of Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein

The purity of Biotinylated Cynomolgus GFRAL is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein

Biotinylated Cynomolgus GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.