ELISA with Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Prote 4281

SDS-PAGE gel of Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex 4281

Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein 4281

$675.00 – $2,250.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)

SKU: 4281parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
molecular weight	The protein has a predicted MW of 70.5 kDa (GARP)&41.3 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF Beta) based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein 4281

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Protein names Leucine rich repeat containing 32
Gene names LRRC32,LRRC32
Mass 9541Da
Target Relevance information above includes information from UniProt accession: A0A2K5X2X9
The UniProt Consortium

Data

ELISA with Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Prote

Immobilized Biotinylated Cynomolgus GARP&Latent TGF Beta 1 Complex, His Tag at 2µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.

HPLC of Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein

The purity of Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex

Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.