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Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) 5011

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Binding assay: Yes (SPR)
  • Amino Acid Range: Ile21-Thr503
SKU: 5011parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

molecular weight

The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-70 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) 5011

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

SPR with Biotinylated Cynomolgus Alkaline Phosphatase (Placental type)
Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Biotinylated Cynomolgus ALPP, His Tag with an affinity constant of 82.54 nM as determined in SPR assay (Biacore T200).
HPLC of Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /
The purity of Biotinylated Cynomolgus ALPP is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Cynomolgus Alkaline Phosphatase (Placental
Biotinylated Cynomolgus ALPP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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