ELISA with Rhesus macaque Complement Factor D/CFD Protein 5161

Rhesus macaque Complement Factor D/CFD Protein 5161

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln21-Ala253

SKU: 5161parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	H9EXC1
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Complement factor D is a serine protease essential for the activation of the alternative pathway and is expressed in the kidney, adipocytes, and macrophages. Factor D is found at relatively high levels in glomeruli suggesting that this component of the complement cascade could influence renal pathophysiology.Complement factor D or alternative pathway activation is needed to prevent spontaneous accumulation of C3 and IgM deposits within the mesangium.
molecular weight	The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 26-30 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Rhesus macaque Complement Factor D/CFD Protein 5161

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Complement factor D is a serine protease essential for the activation of the alternative pathway and is expressed in the kidney, adipocytes, and macrophages. Factor D is found at relatively high levels in glomeruli suggesting that this component of the complement cascade could influence renal pathophysiology.Complement factor D or alternative pathway activation is needed to prevent spontaneous accumulation of C3 and IgM deposits within the mesangium.
Protein names Complement factor D (EC 3.4.21.46) (Adipsin) (C3 convertase activator) (Properdin factor D)
Gene names CFD,CFD
Mass 27262Da
Function Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Catalytic activity CATALYTIC ACTIVITY: Reaction=Selective cleavage of Arg-\|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.; EC=3.4.21.46; Evidence=;
Target Relevance information above includes information from UniProt accession: H9EXC1
The UniProt Consortium

Data

ELISA with Rhesus macaque Complement Factor D/CFD Protein

Immobilized Rhesus macaque Complement Factor D, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA.

HPLC of Rhesus macaque Complement Factor D/CFD Protein

The purity of Rhesus macaque Complement Factor D is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Rhesus macaque Complement Factor D/CFD Protein

Rhesus macaque Complement Factor D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.